The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 20, 2021

Filed:

Apr. 28, 2017
Applicants:

Daniel Ory, Saint Louis, MO (US);

Xuntian Jiang, Saint Louis, MO (US);

Inventors:

Daniel Ory, Saint Louis, MO (US);

Xuntian Jiang, Saint Louis, MO (US);

Assignee:

Washington University, St. Louis, MO (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/53 (2006.01); G01N 33/68 (2006.01); G01N 33/92 (2006.01); A61K 31/575 (2006.01); G01N 33/74 (2006.01); G01N 30/72 (2006.01); C12M 3/00 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5308 (2013.01); A61K 31/575 (2013.01); G01N 33/6848 (2013.01); G01N 33/743 (2013.01); G01N 33/92 (2013.01); C12M 3/00 (2013.01); G01N 30/7233 (2013.01); G01N 2800/04 (2013.01); G01N 2800/085 (2013.01); G01N 2800/28 (2013.01); G01N 2800/38 (2013.01);
Abstract

Methods for identification and quantification of bile acids are disclosed. Bile acids in plasma, serum and/or blood such as a dried blood spot are used to identify subjects with a Niemann-Pick disease. The methods include measuring levels of a bile acid, such as 3β,5α,6β-trihydroxycholanic acid, N-(3β,5α,6β-trihydroxy-cholan-24-oyl)glycine, N-(3β,5α,6β-trihydroxy-cholan-24-oyl)taurine, or a combination thereof. Detection of bile acids involve mass spectroscopy and/or a combination of mass spectroscopy and liquid chromatography such as a LC-MS/MS assay. The methods can be used with sphingomyelinase assays to detect, diagnose and differentiate between Niemann-Pick A/B and Niemann-Pick C (NPC) disease.


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