The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 20, 2021

Filed:

Nov. 29, 2018
Applicant:

Xgenomes Corp., Boston, MA (US);

Inventor:

Kalim Mir, Boston, MA (US);

Assignee:

XGENOMES CORP., Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6809 (2018.01); G01N 33/53 (2006.01); C12N 15/10 (2006.01); G16B 30/00 (2019.01); G16B 40/20 (2019.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6809 (2013.01); C12N 15/10 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); G01N 33/5308 (2013.01); G16B 30/00 (2019.02); G16B 40/20 (2019.02);
Abstract

Systems and methods for nucleic acid sequencing are provided. Nucleic acid is fixed in double-stranded linearized stretched form on a test substrate before being denatured into to single stranded form on the substrate to obtain adjacent fixed first and second strands of the nucleic acid. The strands are exposed to a respective pool of a respective oligonucleotide probe in a set of probes under conditions allowing for probes to form a heteroduplex with a corresponding complementary portion of the fixed first or second strand thereby giving rise to a respective instance of optical activity. An imager measures a location and duration on the substrate of this optical activity. The exposing and measuring is repeated for probes in the set of probes thereby obtaining a plurality of sets of positions. The nucleic acid sequence is determined from the plurality of sets of positions through compilation of the positions in the sets.


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