The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 20, 2021
Filed:
May. 07, 2018
Applicant:
Old Dominion University Research Foundation, Norfolk, VA (US);
Inventors:
Olga Pakhomova, Norfolk, VA (US);
Andrei G. Pakhomov, Norfolk, VA (US);
Assignee:
Old Dominion University Research Foundation, Norfolk, VA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 13/00 (2006.01); A61N 1/32 (2006.01);
U.S. Cl.
CPC ...
C12N 13/00 (2013.01); A61N 1/327 (2013.01);
Abstract
Methods of enhancing membrane permeabilization in a cell are provided. A method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the method, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm.