The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 13, 2021

Filed:

Aug. 28, 2017
Applicant:

United States of America As Represented BY the Administrator of Nasa, Washington, DC (US);

Inventors:

Travis David Boone, Mountain View, CA (US);

Jimmy Kar Chuen Jung, Mountain View, CA (US);

Macarena Parra, Gilroy, CA (US);

Mark Brown, Yorba Linda, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/686 (2018.01); B01D 61/18 (2006.01); C12N 15/09 (2006.01); C12P 19/34 (2006.01); G01N 33/53 (2006.01); G05D 7/06 (2006.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); B01D 61/18 (2013.01); C12N 15/09 (2013.01); C12N 15/1003 (2013.01); C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); G01N 33/53 (2013.01); G05D 7/0629 (2013.01);
Abstract

A novel assay and a suite of devices may isolate nucleic acids from prokaryotic and eukaryotic cells and prepare samples for real-time (quantitative) polymerase chain reaction (PCR) analysis. The assay may employ an aqueous-based non-alcohol approach that yields robust RNA quality. The suite of ready-to-use devices may provide pre-loaded reagents in liquid and lyophilized formats to enable rapid manual operation in a laboratory or remote field environments. The assay and devices may be particularly suitable to analysis in microgravity or deep space environments.


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