The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 13, 2021
Filed:
Jan. 23, 2019
Applicant:
Jiangnan University, Wuxi, CN;
Inventors:
Assignee:
JIANGNAN UNIVERSITY, Wuxi, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/75 (2006.01); C07K 14/435 (2006.01);
U.S. Cl.
CPC ...
C12N 15/75 (2013.01); C07K 14/43504 (2013.01);
Abstract
The present disclosure relates to a method for regulating expression of protein of interest in, and belongs to the technical field of genetic engineering. The method comprises: usingas an expression host, adding the N-terminal nucleotide sequence coding the first 15 amino acids of the endogenous protein before the coding gene of the protein of interest or modifying the original N-terminal nucleotide sequence coding the first 15 amino acids, and performing free expression in plasmid, thereby regulating expression of the protein of interest in, and even regulating the expression difference in different growth phases and the expression level.