The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 13, 2021

Filed:

Sep. 24, 2014
Applicant:

ID Pharma Co., Ltd., Tokyo, JP;

Inventors:

Hiroshi Ban, Ibaraki, JP;

Akihiro Iida, Ibaraki, JP;

Mamoru Hasegawa, Ibaraki, JP;

Assignee:

ID Pharma Co., Ltd., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/074 (2010.01); A61K 35/545 (2015.01); C12N 7/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); A61K 35/545 (2013.01); C12N 7/00 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/606 (2013.01); C12N 2506/09 (2013.01); C12N 2510/00 (2013.01); C12N 2523/00 (2013.01); C12N 2760/18843 (2013.01); C12N 2830/00 (2013.01); C12N 2840/00 (2013.01);
Abstract

The present invention provides methods for improving the efficiency of inducing pluripotent stem cells, as well as vectors and compositions for use therein. In the induction of pluripotent stem cells which contains the step of introducing a vector that contains the KLF gene, OCT gene, and SOX gene in this order, the efficiency of pluripotent stem cell induction was successfully increased significantly by further introducing a vector that contains the KLF gene but not the OCT gene and the SOX gene. The methods of the present invention have an excellent feature in that they allow efficient induction of pluripotent stem cells under a temperature condition closer to the physiological environment, and prompt vector removal after the pluripotent stem cell induction. The present invention enables more efficient induction of pluripotent stem cells.


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