The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 13, 2021
Filed:
Dec. 12, 2019
Applicant:
Biomadison, Inc, Del Mar, CA (US);
Inventors:
Ward C Tucker, Monona, WI (US);
Francis Mark Dunning, Madison, WI (US);
Assignee:
BioMadison, Inc., Del Mar, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/00 (2006.01); C12Q 1/37 (2006.01); G01N 33/542 (2006.01); C12N 9/52 (2006.01);
U.S. Cl.
CPC ...
C07K 14/00 (2013.01); C12N 9/52 (2013.01); C12Q 1/37 (2013.01); G01N 33/542 (2013.01); G01N 2333/33 (2013.01);
Abstract
Reporting constructs for characterizingneurotoxin protease activity and suitable for use in a cell-based assay are provided. The reporting construct can be a single recombinant hybrid protein or a pair of recombinant hybrid proteins that act in concert. The recombinant hybrid protein(s) include a fluorophore and an N-terminal non-peptide membrane anchoring portion. The recombinant hybrid protein or at least one of a pair of recombinant hybrid proteins that act in concert include aneurotoxin protease recognition and cleavage sequence positioned to release a fluorophore upon cleavage.