The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 2021

Filed:

Jun. 14, 2018
Applicant:

The Trustees of Columbia University IN the City of New York, New York, NY (US);

Inventors:

Jonathan A Javitch, Dobbs Ferry, NY (US);

Maria Hauge Pedersen, Brooklyn, NY (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/566 (2006.01); C12N 9/12 (2006.01);
U.S. Cl.
CPC ...
G01N 33/566 (2013.01); C12N 9/12 (2013.01); C12Y 207/11016 (2013.01); G01N 2333/4703 (2013.01); G01N 2333/705 (2013.01); G01N 2500/02 (2013.01);
Abstract

The current invention is methods and assays for detecting and/or measuring the modification, i.e., activation or inhibition, of a receptor of interest, without the need to modify the receptor with a label or a tag. The invention uses proteins that move, translocate, are recruited and/or bind to the receptor of interest when the receptor is modified, and proteins in proximity to and/or in the same compartment as the receptor of interest. The proteins are attached or linked to polypeptides that result from the unique cleavage of a modifiedluciferase. When the proteins are in proximity allowing the unique polypeptides to recombine, a luminescent signal is generated. The invention also includes vector encoding these proteins, cells expressing these proteins, compositions, system and kits.


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