The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 30, 2021
Filed:
Jun. 16, 2020
Biofidelity Ltd, Cambridge, GB;
Barnaby Balmforth, Cambridge, GB;
Cameron Frayling, Cambridge, GB;
Ana Silva-Weatherley, Cambridge, GB;
Magdalena Stolarek-Januszkiewicz, Cambridge, GB;
BIOFIDELITY LTD, Cambridge, GB;
Abstract
A method of detecting a target polynucleotide sequence in a given nucleic acid analyte characterised by the steps of: a. annealing the analyte to a single-stranded probe oligonucleotide Ato create a first intermediate product which is at least partially double-stranded and in which the 3' end of Aforms a double-stranded complex with the analyte target sequence; b. pyrophosphorolysing the first intermediate product with a pyrophosphorolysing enzyme in the 3′-5′ direction from the 3′ end of Ato create partially digested strand Aand the analyte; c. (i) annealing Ato a single-stranded trigger oligonucleotide B and extending the Astrand in the 5′-3′ direction against B; or (ii) circularising Athrough ligation of its 3′ and 5′ ends; or (iii) ligating the 3′ end of Ato the 5′ end of a ligation probe oligonucleotide C; in each case to create an oligonucleotide A; d. priming Awith at least one single-stranded primer oligonucleotide and creating multiple copies of A, or a region of A; and e. detecting a signal derived from the multiple copies and inferring therefrom the presence or absence of the polynucleotide target sequence in the analyte.