The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 2021

Filed:

May. 22, 2015
Applicant:

University of Houston System, Houston, TX (US);

Inventors:

Wei-Chuan Shih, Houston, TX (US);

Richard Willson, Houston, TX (US);

Assignee:

UNIVERSITY OF HOUSTON SYSTEM, Houston, TX (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6816 (2018.01); G01N 21/65 (2006.01); G01N 21/64 (2006.01); G01N 21/552 (2014.01); G01N 33/543 (2006.01); G01N 33/553 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6816 (2013.01); G01N 21/554 (2013.01); G01N 21/648 (2013.01); G01N 21/658 (2013.01); G01N 33/54373 (2013.01); G01N 33/553 (2013.01);
Abstract

A methodology for assays and diagnostics utilizes a nanoporous or corrugated metal-containing surface, fiber or particle which enhances or suppresses the optical detectability of a label. The resulting optical, electromagnetic, or imaging signal signals the presence of a pathogen or analyte of interest. Preferred embodiments pertain to label-free, in situ monitoring of individual DNA hybridization in microfluidics using molecular sentinel probes immobilized on nanoporous gold disks. By immobilizing molecular sentinel probes on nanoporous gold disks, single-molecule sensitivity is demonstrated via surface-enhanced Raman scattering which provides robust signals. The described methodology is generally applicable to most amplification independent assays and molecular diagnostics.


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