The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 30, 2021

Filed:

Apr. 28, 2016
Applicants:

Politecnico Di Milano, Milan, IT;

Fondazione Cariplo, Milan, IT;

Inventors:

Alberto Redaelli, Milan, IT;

Marco Rasponi, Milan, IT;

Paola Occhetta, Romentino, IT;

Assignees:

POLITECNICO DI MILANO, Milan, IT;

FONDAZIONE CARIPLO, Milan, IT;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 3/06 (2006.01); C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/42 (2006.01);
U.S. Cl.
CPC ...
C12M 23/16 (2013.01); C12M 21/08 (2013.01); C12M 23/32 (2013.01); C12M 23/34 (2013.01); C12M 25/02 (2013.01); C12M 35/02 (2013.01); C12M 35/04 (2013.01);
Abstract

A microfluidic device for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs that includes a culture chamber may include: a confinement apparatus configured to define at least one compartment configured to contain a cellular matrix and at least one compartment configured to contain a culture medium, the confinement apparatus being hydrophobic and pervious to the culture medium; and/or at least one counter element. The confinement apparatus and at least one counter element may be reciprocally mobile between resting and compression positions of the cellular matrix. A method for controlled generation and/or culture and/or maturation of three-dimensional cells and/or tissue constructs at a microscale may include: controlled compression of a cellular matrix for a predetermined period of time. The cellular matrix may be delimited by a confinement apparatus that is pervious to a culture medium.


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