The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 23, 2021

Filed:

Feb. 23, 2018
Applicant:

Oxford University Innovation Limited, Oxford, GB;

Inventors:

Carol Robinson, Oxford, GB;

Hsin-Yung Yen, Oxford, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
H01J 49/00 (2006.01); G01N 33/68 (2006.01); C07F 9/09 (2006.01); H01J 49/16 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); C07F 9/091 (2013.01); H01J 49/165 (2013.01); G01N 2333/726 (2013.01); G01N 2560/00 (2013.01);
Abstract

A method of detecting a membrane protein by mass spectrometry comprises: (a) providing a solution comprising a detergent micelle in which said membrane protein is contained; (b) providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; (c) vaporising the solution using the nanoelectrospray ionisation source under conditions such that the membrane protein is released from the micelle; (d) ionising the membrane protein; (e) resolving the ionised membrane protein using the mass analyser; and (f) detecting the resolved membrane protein using the detector; wherein the solution contains a phosphate ester detergent which forms said detergent micelle. Also provided are reagents for use in said method.


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