The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 23, 2021
Filed:
Oct. 19, 2016
Applicant:
Roche Innovation Center Copenhagen A/s, Horsholm, DK;
Inventors:
Franziska Boess, Basel, CH;
Sabine Sewing, Basel, CH;
Annie Moisan, Basel, CH;
Adrian B. Roth, Basel, CH;
Cristina Bertinetti-Lapatki, Basel, CH;
Assignee:
Roche Innovation Center Copenhagen A/S, Basel, CH;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/50 (2006.01); C12N 15/11 (2006.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
G01N 33/5014 (2013.01); C12N 5/067 (2013.01); C12N 15/111 (2013.01); C12N 2310/3231 (2013.01); C12N 2320/10 (2013.01); C12N 2320/53 (2013.01);
Abstract
The invention relates to methods for predicting the in vivo toxicity of oligonucleotides, such as antisense oligonucleotides using in vitro cell based assays based on gymnotically administering oligonucleotides to primary mammalian hepatocytes and subsequently measuring the levels of toxicity biomarkers such as the release of LDH into the cell culture media and/or intracellular ATP.