The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 23, 2021

Filed:

Jan. 12, 2017
Applicant:

Agency for Science, Technology and Research, Singapore, SG;

Inventors:

Tianxun Gong, Singapore, SG;

Malini Olivo, Singapore, SG;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 21/65 (2006.01); G01N 33/53 (2006.01); G01N 33/48 (2006.01); C07C 233/02 (2006.01); G02B 6/02 (2006.01); B82Y 20/00 (2011.01);
U.S. Cl.
CPC ...
G01N 21/658 (2013.01); C07C 233/02 (2013.01); G01N 33/5308 (2013.01); B82Y 20/00 (2013.01); G01N 33/48 (2013.01); G01N 2021/656 (2013.01); G02B 6/0229 (2013.01); Y10T 436/24 (2015.01);
Abstract

A method for assessing a state of a living cell using surface enhanced Raman spectroscopy (SERS) is provided. The method may include modifying one or more living eel Is with an alkyne-containing compound to form one or more modified living cells, mixing the one or more modified living cells with a SERS-active material to form a mixture, injecting the mixture into a conduit defined by an inner wall of a hollow core photonic crystal fiber, and detecting a surface enhanced Raman signal from the mixture in the conduit. In preferred embodiments, the alkyne containing compound is linoleamide alkyne (LLA) for the detection of lipid peroxidation or 4-(dihydroxyborophenyl) acetylene (DBA) for the detection of sialic acid expression in cells, both using gold nanoparticles as the SERS-active material.


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