The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 23, 2021

Filed:

Dec. 20, 2017
Applicants:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Essex, GB;

Inventors:

James Tsay, San Diego, CA (US);

Anmiv Prabhu, San Diego, CA (US);

David Heiner, Vista, CA (US);

Edwin Li, Los Angeles, CA (US);

Alexandre Richez, Cambridge, GB;

John M. Beierle, Carlsbad, CA (US);

Kevan Samiee, San Diego, CA (US);

Kristina Munoz, Coronado, CA (US);

Leonid Malevanchik, San Diego, CA (US);

Ludovic Vincent, San Diego, CA (US);

Naiqian Zhan, San Diego, CA (US);

Peyton Shieh, San Diego, CA (US);

Robert Yang, San Diego, CA (US);

Samantha Schmitt, San Diego, CA (US);

Sang Park, San Diego, CA (US);

Scott Bailey, San Diego, CA (US);

Sean M. Ramirez, San Diego, CA (US);

Sunmin Ahn, San Diego, CA (US);

Valerie Uzzell, Solana Beach, CA (US);

Wei Wei, San Diego, CA (US);

Yuxiang Huang, San Diego, CA (US);

Tyler Jamison Dill, San Diego, CA (US);

Assignees:

Illumina, Inc., San Diego, CA (US);

Illumina Cambridge Limited, Cambridge, GB;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 15/14 (2006.01); C12Q 1/6874 (2018.01); B01L 3/00 (2006.01); C09D 133/24 (2006.01); C09D 133/26 (2006.01); B01J 19/00 (2006.01); G01N 15/10 (2006.01);
U.S. Cl.
CPC ...
G01N 15/1436 (2013.01); B01J 19/0046 (2013.01); B01L 3/502 (2013.01); B01L 3/502707 (2013.01); C09D 133/24 (2013.01); C09D 133/26 (2013.01); C12Q 1/6874 (2013.01); G01N 15/1484 (2013.01); B01J 2219/00286 (2013.01); B01J 2219/00317 (2013.01); B01J 2219/00319 (2013.01); B01J 2219/00418 (2013.01); B01J 2219/00585 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00612 (2013.01); B01J 2219/00637 (2013.01); B01J 2219/00722 (2013.01); B01L 2200/12 (2013.01); B01L 2300/087 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0848 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/16 (2013.01); G01N 2015/1006 (2013.01);
Abstract

A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.


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