The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 23, 2021

Filed:

Apr. 11, 2016
Applicant:

Icosagen Cell Factory Oü, Tartumaa, EE;

Inventors:

Mart Ustav, Össu, EE;

Ene Ustav, Össu, EE;

Andres Männik, Össu, EE;

Mart Toots, Össu, EE;

Mart Ustav, Jr., Össu, EE;

Andres Tover, Össu, EE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/11 (2006.01); A61K 38/00 (2006.01); A61K 48/00 (2006.01); C07K 14/705 (2006.01); C12N 15/86 (2006.01); C12Q 1/18 (2006.01); C12Q 1/70 (2006.01); C12N 5/09 (2010.01);
U.S. Cl.
CPC ...
C12Q 1/18 (2013.01); C12N 5/0693 (2013.01); C12N 15/86 (2013.01); C12Q 1/706 (2013.01); C12Q 1/708 (2013.01); C12N 2503/02 (2013.01); C12N 2510/00 (2013.01); C12N 2710/16621 (2013.01); C12N 2710/16622 (2013.01); C12N 2710/20011 (2013.01); C12Q 2600/136 (2013.01); G01N 2333/025 (2013.01); G01N 2500/10 (2013.01);
Abstract

A U2OS-based model system using luciferase reporters to monitor the genome replication of alpha and beta HPVs is provided. A modified U2OS cell line is disclosed that expresses Firefly luciferase to measure toxicity of the screened compounds. In addition, provided are HPV18, HPV 16 and HPV5 marker genomes that expressluciferase under the control of viral promoters used to measure changes in the viral copy number. This ready-to-use model system is capable of being used in high-throughput screens to identify compounds inhibiting initial amplification and stable maintenance as well as vegetative phase of various HPV subtypes.


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