The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 16, 2021

Filed:

Mar. 17, 2020
Applicant:

Bio-rad Laboratories, Inc., Hercules, CA (US);

Inventors:

Kalyan Handique, Ann Arbor, MI (US);

John Stark, Ann Arbor, MI (US);

Yadwinder Deol, Ann Arbor, MI (US);

Vishal Sharma, Ann Arbor, MI (US);

Assignee:

Bio-Rad Laboratories, Inc., Hercules, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6811 (2018.01); C12Q 1/6874 (2018.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6811 (2013.01); C12N 15/1065 (2013.01); C12Q 1/6874 (2013.01); C12Q 2563/179 (2013.01); C12Q 2565/629 (2013.01); C12Q 2600/178 (2013.01);
Abstract

A system and method for target material capture, the method comprising: receiving a set of target cells into an array of wells defined at a surface plane of a substrate; receiving a set of particles into the array of wells, thereby co-capturing the set of target cells and the set of particles; achieving a desired state for the array of wells upon receiving a washing fluid into a cavity in communication with the array of wells; receiving a lysis buffer into the cavity; receiving a partitioning fluid into the cavity, thereby displacing the lysis buffer from the cavity and partitioning each of the array of wells from adjacent wells, at the surface plane; and retaining intercellular material of the set of target cells, individually with the set of particles within the array of wells.


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