The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 16, 2021
Filed:
Apr. 24, 2019
Applicant:
Battelle Memorial Institute, Richland, WA (US);
Inventors:
Assignee:
Battelle Memorial Institute, Richland, WA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/15 (2006.01); C12N 9/88 (2006.01); C12N 9/10 (2006.01); C12N 9/04 (2006.01); C12P 7/42 (2006.01); C12P 7/52 (2006.01); C12N 15/80 (2006.01); C12P 7/48 (2006.01);
U.S. Cl.
CPC ...
C12N 15/80 (2013.01); C12N 9/0006 (2013.01); C12N 9/1096 (2013.01); C12N 9/88 (2013.01); C12P 7/42 (2013.01); C12P 7/48 (2013.01); C12Y 101/01059 (2013.01); C12Y 206/01018 (2013.01); C12Y 401/01011 (2013.01);
Abstract
This application provides recombinantfungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.