The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 16, 2021

Filed:

Oct. 20, 2016
Applicant:

Fujifilm Cellular Dynamics, Inc., Madison, WI (US);

Inventors:

Maksym A. Vodyanyk, Madison, WI (US);

Xin Zhang, Madison, WI (US);

Andrew J. Brandl, Madison, WI (US);

Deepika Rajesh, Madison, WI (US);

Bradley Swanson, Madison, WI (US);

Christie Munn, Madison, WI (US);

Sarah A. Burton, Madison, WI (US);

Wen Bo Wang, Madison, WI (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0783 (2010.01); C12N 5/0784 (2010.01); C12N 5/0789 (2010.01); C12N 5/0781 (2010.01); C12N 15/85 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0636 (2013.01); C12N 5/0635 (2013.01); C12N 5/0637 (2013.01); C12N 5/0638 (2013.01); C12N 5/0639 (2013.01); C12N 5/0646 (2013.01); C12N 5/0647 (2013.01); C12N 15/85 (2013.01); C12N 15/86 (2013.01); C12N 2500/38 (2013.01); C12N 2500/90 (2013.01); C12N 2501/115 (2013.01); C12N 2501/125 (2013.01); C12N 2501/145 (2013.01); C12N 2501/155 (2013.01); C12N 2501/165 (2013.01); C12N 2501/22 (2013.01); C12N 2501/2303 (2013.01); C12N 2501/2306 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/25 (2013.01); C12N 2501/26 (2013.01); C12N 2501/60 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2501/727 (2013.01); C12N 2501/998 (2013.01); C12N 2501/999 (2013.01); C12N 2506/025 (2013.01); C12N 2506/03 (2013.01); C12N 2506/09 (2013.01); C12N 2506/11 (2013.01); C12N 2506/45 (2013.01); C12N 2510/00 (2013.01); C12N 2533/50 (2013.01); C12N 2840/20 (2013.01);
Abstract

Provided herein are methods for the efficient in vitro differentiation of somatic cell-derived pluripotent stem cells to hematopoietic precursor cells, and the further differentiation of the hematopoietic precursor cells into immune cells of various myeloid or lymphoid lineages, particularly T cells, NK cells, and dendritic cells. The pluripotent cells may be maintained and differentiated under defined conditions; thus, the use of mouse feeder cells or serum is not required in certain embodiments for the differentiation of the hematopoietic precursor cells.


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