The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 16, 2021

Filed:

Aug. 29, 2017
Applicant:

Flodesign Sonics, Inc., Wilbraham, MA (US);

Inventors:

Bart Lipkens, Hampden, MA (US);

Rudolf Gilmanshin, Framingham, MA (US);

Louis Masi, Wilbraham, MA (US);

Benjamin Ross-Johnsrud, Wilbraham, MA (US);

Erik Miller, Belchertown, MA (US);

Walter M. Presz, Jr., Wilbraham, MA (US);

Thomas J. Kennedy, III, Wilbraham, MA (US);

Assignee:

FloDesign Sonics, Inc., Wilbraham, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 21/28 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); G10K 15/04 (2006.01); G10K 9/122 (2006.01); C12M 1/26 (2006.01); C12N 13/00 (2006.01); C07K 1/14 (2006.01); C12M 1/42 (2006.01); B01D 17/06 (2006.01); B06B 1/06 (2006.01); B01D 17/04 (2006.01); G10K 15/00 (2006.01);
U.S. Cl.
CPC ...
C12M 47/02 (2013.01); B01D 17/04 (2013.01); B01D 17/06 (2013.01); B01D 21/283 (2013.01); B06B 1/0644 (2013.01); C07K 1/14 (2013.01); C12M 29/10 (2013.01); C12M 29/18 (2013.01); C12M 33/08 (2013.01); C12M 35/04 (2013.01); C12M 41/12 (2013.01); C12M 47/10 (2013.01); C12N 13/00 (2013.01); G10K 9/122 (2013.01); G10K 15/00 (2013.01); G10K 15/043 (2013.01);
Abstract

Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.


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