The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 16, 2021
Filed:
Jun. 13, 2017
Applicant:
Biogen MA Inc., Cambridge, MA (US);
Inventors:
Robert S. Gronke, Cambridge, MA (US);
Ratnesh S. Joshi, Cambridge, MA (US);
Assignee:
BIOGEN MA INC., Cambridge, MA (US);
Primary Examiner:
Int. Cl.
CPC ...
C07H 1/06 (2006.01); C07H 1/00 (2006.01); B01D 15/30 (2006.01); B01D 15/42 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
C07H 1/06 (2013.01); B01D 15/305 (2013.01); B01D 15/426 (2013.01); C07H 1/00 (2013.01); C07H 21/02 (2013.01); C07H 21/04 (2013.01);
Abstract
The invention herein describes a method to purify a target oligonucleotide using hydrophobic interaction chromatography (HIC). The method includes adding a salt to a mixture of the target oligonucleotide and product-related impurities, applying the diluted mixture, at a particular dynamic loading capacity, to the hydrophobic interaction chromatography resin (or hydrophobic adsorbent), washing the hydrophobic adsorbent with an aqueous salt solution, eluting the target oligonucleotide with a eluting solution, and collecting the eluent comprising the target oligonucleotide.