The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10942127 B1

PDF
Full Text
Expired
Date of Patent:
Mar. 09, 2021

Filed:

Oct. 20, 2014
Applicant:

Osaka University, Osaka, JP;

Inventors:

Hiroyuki Yoshikawa, Osaka, JP;

Eiichi Tamiya, Osaka, JP;

Shuhei Imura, Osaka, JP;

Assignee:

OSAKA UNIVERSITY, Osaka, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/26 (2006.01); G01N 21/17 (2006.01); G01N 21/82 (2006.01); G01N 33/58 (2006.01); G01N 33/535 (2006.01); G01N 21/78 (2006.01); G01N 21/77 (2006.01);
U.S. Cl.
CPC ...
G01N 21/82 (2013.01); C12Q 1/26 (2013.01); G01N 21/17 (2013.01); G01N 21/1717 (2013.01); G01N 21/78 (2013.01); G01N 33/535 (2013.01); G01N 33/581 (2013.01); G01N 2021/1725 (2013.01); G01N 2021/7773 (2013.01); G01N 2201/062 (2013.01); G01N 2201/06113 (2013.01); G01N 2333/904 (2013.01);
Abstract

The present invention provide a method for detecting a test substance utilizing a color development caused by an enzyme reaction, or a color development caused by a specific interaction such as an antigen-antibody reaction and an enzyme reaction, the test substance being detected rapidly, sensitively and quantitatively without using a spectroscopic measurement device. The method for measuring a concentration of a test substance by the present invention comprises the steps of: generating a peroxide from the test substance; obtaining a polymerized substance by bringing an oxidoreductase for producing a polymerized substance and a substrate of the oxidoreductase for producing a polymerized substance into contact with the peroxide; and irradiating the polymerized substance with light to record a temporal variation information of an intensity of scattered light generated from an irradiation point.


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