The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 09, 2021

Filed:

Mar. 18, 2019
Applicant:

Pacific Biosciences of California, Inc., Menlo Park, CA (US);

Inventors:

Jonas Korlach, Camas, WA (US);

Stephen Turner, Eugene, OR (US);

Lei Sun, San Jose, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); G01N 27/327 (2006.01); G01N 27/00 (2006.01); B82Y 15/00 (2011.01); C12Q 1/00 (2006.01); C40B 60/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); B82Y 15/00 (2013.01); C12Q 1/001 (2013.01); G01N 27/00 (2013.01); G01N 27/3277 (2013.01); G01N 27/3278 (2013.01); C12Q 2521/543 (2013.01); C12Q 2563/113 (2013.01); C12Q 2565/607 (2013.01); C40B 60/10 (2013.01);
Abstract

Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand.


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