The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 09, 2021
Filed:
Oct. 14, 2016
Applicant:
Qiagen Sciences, Llc, Germantown, MD (US);
Inventors:
Yexun Wang, Ellicott City, MD (US);
Quan Peng, Clarksburg, MD (US);
Assignee:
QIAGEN Sciences, LLC, Germantown, MD (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12N 9/12 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/686 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); C12N 9/1252 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6869 (2013.01);
Abstract
The present disclosure provides methods and kits for highly multiplex single primer extensions using a MutS protein and Mg2+ at a concentration higher than that in a typical PCR reaction. Also disclosed is the use of such methods and kits in next generation sequencing.