The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 09, 2021

Filed:

Apr. 21, 2017
Applicant:

Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);

Inventors:

Laimonas Kelbauskas, Chandler, AZ (US);

Honor Glenn, Mesa, AZ (US);

Jeff Houkal, Los Angeles, CA (US);

Clifford Anderson, Tempe, AZ (US);

Yanqing Tian, Tempe, AZ (US);

Fengyu Su, Tempe, AZ (US);

Deirdre Meldrum, Phoenix, AZ (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01L 1/00 (2006.01); C12M 3/06 (2006.01); C12M 1/34 (2006.01); C12Q 1/02 (2006.01); G01N 33/487 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502753 (2013.01); C12M 23/16 (2013.01); C12M 41/46 (2013.01); B01L 2200/0668 (2013.01); B01L 2400/086 (2013.01); C12Q 1/02 (2013.01); G01N 33/48735 (2013.01);
Abstract

A device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation is provided. In certain aspects, an integrated microfluidic device for multi-parameter metabolic and other phenotypic profiling of live biological cells is useable with: 1) multicellular clusters or small biopsy tissue samples, 2) cultures of the constituent cells obtained after cluster/tissue dissociation, and 3) the same constituent single cells in isolation. The approach enables study of the effects of multicellular complexity, such as in response to treatment, pathogens, stress, or other factors concerning disease origination and progression. Measurements may be performed on single cells or multicellular populations or tissues in the same assay at the same time.


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