The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 02, 2021
Filed:
Jun. 18, 2015
Applicants:
Universite Grenoble Alpes, Saint-Martin-d'Hères, FR;
European Molecular Biology Laboratory, Heidelberg, DE;
Inventors:
Assignees:
Universite Grenoble Alpes, Saint Martin d'Heres, FR;
European Molecular Biology Laboratory, Heidelberg, DE;
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/542 (2006.01); C12Q 1/48 (2006.01); C12N 9/12 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
G01N 33/542 (2013.01); C12N 9/12 (2013.01); C12Q 1/485 (2013.01); C12Y 207/11031 (2013.01); G01N 33/5008 (2013.01); G01N 2333/91215 (2013.01); G01N 2500/00 (2013.01); G01N 2500/10 (2013.01);
Abstract
The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.