The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 02, 2021
Filed:
Mar. 26, 2019
South China University of Technology, Guangdong, CN;
Shuizhu Wu, Guangdong, CN;
Lingfeng Xu, Guangdong, CN;
Ling Ni, Guangdong, CN;
Fang Zeng, Guangdong, CN;
SOUTH CHINA UNIVERSITY OF TECHNOLOGY, Guangdong, CN;
Abstract
The present invention relates to a fluorescent probe for detecting nitroreductase and a preparation method and use thereof in enzymatic reactions, belonging to the field of industrial analysis and detection. The fluorescent probe is 3-(4-(2-(4'-(diphenylamino)-3-((4-nitrobenzyl)oxy)-[1, 1′-biphenyl]-4-yl)vinyl)quinolin-1-ium-1-yl)propane-1-sulfonate. The fluorescent probe of the present invention, with the introduction of hydrophilic groups, sulfonate and quinolinium, the probe's hydrophilicity is enhanced, under the enzymatic catalysis of nitroreductase (NTR), 1,6-rearrangement and elimination reaction occurs, and hydroxyl group is generated. Detection and analysis of the NTR in the industrial enzymatic reactions can be realized due to the change of fluorescence which is induced by the intramolecular charge transfer (ICT) effect. This method has such advantages as easy preparation, high yield and being suitable for detecting high concentration of enzyme in the enzymatic reactions, and it shows an extensive application prospect in the field of enzyme-detection in the industrial enzymatic reaction systems.