The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 02, 2021
Filed:
Mar. 17, 2016
Revolugen Limited, Hadfield, Derbyshire, GB;
Stephen John Minter, Hadfield, GB;
Georgios Patsos, Hadfield, GB;
RevoluGen Limited, Derbyshire, GB;
Abstract
Methods of isolating nucleic acids comprising DNA from biological material are disclosed. The methods comprise a lysis step using an aqueous composition which comprises lithium at a concentration of 0.05 to 1.0M, a chelating agent, and a surfactant to produce a lysed composition. The lysed composition is treated with a solid support that is capable of immobilising DNA in the presence of a dissolved chaotropic agent at a concentration of 0.05 to 2M and 25% to 60% by volume of a Calkanol. The support is then washed with a first wash solution containing lithium dissolved in a Calkanol. Subsequently, the support is washed with a liquid comprising at least 80% by volume of a Calkanol. The nucleic acid comprising DNA is then eluted from the support.