The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 02, 2021

Filed:

Apr. 04, 2016
Applicant:

Kikkoman Corporation, Noda, JP;

Inventors:

Atsushi Ichiyanagi, Noda, JP;

Airi Komatsuzaki, Noda, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/06 (2006.01); C12Q 1/26 (2006.01); G01N 33/72 (2006.01); C12Q 1/28 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0032 (2013.01); C12Q 1/26 (2013.01); C12Y 105/03 (2013.01); G01N 33/72 (2013.01); G01N 33/723 (2013.01); C12Q 1/28 (2013.01); G01N 2333/805 (2013.01);
Abstract

This invention provides an amadoriase having improved specific activity on a glycated substrate, compared with conventional amadoriase. Provided is an amadoriase comprising a substitution of the amino acid at the position corresponding to position 64 of the amino acid sequence as shown in SEQ ID NO: 1 with an amino acid selected from the group consisting of glycine, serine, methionine, leucine, threonine, valine, and isoleucine, a method for measurement of HbA1c, and a reagent kit for measurement of HbA1c using such amadoriase. Such method and kit for measurement enable rapid, simple, and accurate quantification of HbA1c.


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