The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 23, 2021
Filed:
Aug. 14, 2008
Arick Brown, Pacifica, CA (US);
Jerome Bill, Jr., San Francisco, CA (US);
Timothy Tully, San Francisco, CA (US);
Christopher Dowd, Belmont, CA (US);
Arick Brown, Pacifica, CA (US);
Jerome Bill, Jr., San Francisco, CA (US);
Timothy Tully, San Francisco, CA (US);
Christopher Dowd, Belmont, CA (US);
GENENTECH, INC., South San Francisco, CA (US);
Abstract
Methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant are described, which methods comprise the sequential steps of: (a) passing the composition through an ion exchange membrane, where the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and the at least one contaminant, and (b) recovering the purified polypeptide from the effluent.