The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

Aug. 10, 2015
Applicant:

Baylor College of Medicine, Houston, TX (US);

Inventors:

Jin Wang, Sugar Land, TX (US);

Xiqian Jiang, Houston, TX (US);

Jianwei Chen, Houston, TX (US);

Assignee:

BAYLOR COLLEGE OF MEDICINE, Houston, TX (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 33/58 (2006.01); G01N 33/68 (2006.01); C07D 311/16 (2006.01); G01N 33/84 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6815 (2013.01); C07D 311/16 (2013.01); G01N 33/582 (2013.01); G01N 33/84 (2013.01); Y10T 436/182 (2015.01);
Abstract

Embodiments of the present disclosure pertain to methods of detecting a thiol in an environment by exposing the environment to a probe molecule that contains a marker and a thiol responsive group. The thiol responsive group reversibly reacts with the thiol in the environment to form a probe-thiol adduct. This in turn causes a ratiometric change in a spectrometric property of the probe molecule and the probe-thiol adduct, which can then be correlated to the presence of the thiol in the environment. The correlation can occur by quantifying the thiol concentration in the environment. In addition, thiol detection can occur in real-time. Further embodiments of the present disclosure pertain to probe molecules that are utilized for detecting a thiol in an environment. In some embodiments, the probe molecule includes a marker and a thiol responsive group. In some embodiments, the probe molecule also includes an organelle targeting moiety.


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