The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

Apr. 23, 2018
Applicant:

Affymetrix, Inc., Carlsbad, CA (US);

Inventors:

Glenn K. Fu, Dublin, CA (US);

Glenn H. Mcgall, Palo Alto, CA (US);

Robert G. Kuimelis, Palo Alto, CA (US);

Jing Hu, San Jose, CA (US);

Pei-Hua Wang, Fremont, CA (US);

Assignee:

AFFYMETRIX, INC., Carlsbad, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C40B 30/04 (2006.01); C12Q 1/6837 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6858 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6876 (2018.01); C40B 50/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6837 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6858 (2013.01); C12Q 1/6874 (2013.01); C12Q 1/6876 (2013.01); C12Q 2600/106 (2013.01); C12Q 2600/154 (2013.01); C12Q 2600/156 (2013.01); C40B 30/04 (2013.01); C40B 50/06 (2013.01);
Abstract

Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.


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