The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

Mar. 24, 2016
Applicants:

Stichting Wageningen Research, Wageningen, NL;

Technische Universiteit Delft, Delft, NL;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/64 (2006.01); C12N 9/88 (2006.01); C12P 7/42 (2006.01); C12N 9/20 (2006.01); C11C 1/00 (2006.01);
U.S. Cl.
CPC ...
C12P 7/6436 (2013.01); C11C 1/002 (2013.01); C12N 9/20 (2013.01); C12N 9/88 (2013.01); C12P 7/42 (2013.01); C12P 7/64 (2013.01); C12P 7/6418 (2013.01); C12Y 402/01053 (2013.01);
Abstract

It has been found that esterification of a hydroxy-fatty acid by a lipase can be coupled with oleate hydratase (OHase) generation of that hydroxy-FA from an unsaturated FA with a cis C9-C10 double bond, e.g. oleic acid, in a single aqueous buffered reaction medium at low temperature, e.g. 30° C. A simple one-pot enzymatic method to produce fatty acid estolides from one or more triglycerides, e.g. starting from a natural plant oil, is thereby enabled in which the same lipase catalyses both the initial hydrolysis of triglyceride and the final esterification step.


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