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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10920235 B1

PDFFull Text
Date of Patent:
Feb. 16, 2021

Filed:

Dec. 20, 2019

Apparatus for the preparation and use of plant embryo explants for transformation

Applicant:

Monsanto Technology Llc, St. Louis, MO (US);

Inventors:

Beth Jo Calabotta, University City, MO (US);

Kevin Lee Deppermann, Saint Charles, MO (US);

Erik D. Dersch, Fitchburg, WI (US);

Jerald D. Heise, St. Louis, MO (US);

Angela Ranae Koestel, St. Louis, MO (US);

Cindy L. Ludwig, St. Louis, MO (US);

Brian J. Martinell, Mt. Horeb, WI (US);

Edward Williams, Madison, WI (US);

Assignee:

Monsanto Technology LLC, St. Louis, MO (US);

Attorney:

Dentons US LLP

Primary Examiner:

Annette H Para

Int. Cl.
CPC ...
B26D 3/18 (2006.01); B26D 7/06 (2006.01); B26D 7/18 (2006.01); C12N 15/82 (2006.01); C12N 5/00 (2006.01); C12N 9/10 (2006.01); C12R 1/41 (2006.01); A01H 6/20 (2018.01); A01H 6/54 (2018.01); A01H 6/46 (2018.01); A01H 6/60 (2018.01);
U.S. Cl.
CPC ...
C12N 15/8209 (2013.01); C12N 5/0025 (2013.01); C12N 9/1029 (2013.01); C12N 15/8201 (2013.01); C12N 15/8205 (2013.01); C12N 15/8221 (2013.01); C12N 15/8265 (2013.01); C12N 15/8274 (2013.01); C12N 15/8275 (2013.01); C12N 15/8277 (2013.01); A01H 6/202 (2018.05); A01H 6/4684 (2018.05); A01H 6/542 (2018.05); A01H 6/604 (2018.05); C12N 15/8261 (2013.01); C12R 1/41 (2013.01); C12Y 203/01081 (2013.01);
Abstract

The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

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