The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 16, 2021
Filed:
Jun. 07, 2019
Sumitomo Rubber Industries, Ltd., Kobe, JP;
Yukino Inoue, Kobe, JP;
Haruhiko Yamaguchi, Kobe, JP;
Teruhiko Terakawa, Yokohama, JP;
Tsubasa Yano, Yokohama, JP;
SUMITOMO RUBBER INDUSTRIES, LTD., Kobe, JP;
Abstract
Provided is a method that can efficiently produce a transgenicplant in a short period. A method of producing a transgenicplant, including: an infection step of infecting a tissue fragment from aplant with ancontaining a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.