The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

May. 18, 2015
Applicant:

Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, CN;

Inventors:

Yong Wang, Shanghai, CN;

Zhiqiang Xiong, Shangai, CN;

Shujie Song, Shanghai, CN;

Qiaoxia Liu, Shanghai, CN;

Jianfeng Wang, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/70 (2006.01); C12N 15/113 (2010.01); C12N 9/12 (2006.01); C12P 19/62 (2006.01); C12P 17/08 (2006.01); C07H 17/08 (2006.01); C12N 15/09 (2006.01); C12Q 1/18 (2006.01); C12R 1/465 (2006.01);
U.S. Cl.
CPC ...
C12N 15/70 (2013.01); C07H 17/08 (2013.01); C12N 9/1288 (2013.01); C12N 15/09 (2013.01); C12N 15/113 (2013.01); C12P 17/08 (2013.01); C12P 19/62 (2013.01); C12Q 1/18 (2013.01); C12R 1/465 (2013.01); C12N 2320/12 (2013.01); C12N 2330/31 (2013.01); C12N 2330/50 (2013.01);
Abstract

The present invention relates to a method for improving the heterologous synthesis of a polyketide byand use thereof. The yield of the polyketide heterologously synthesized byis significantly increased by attenuating the expression of seventy-two genes, such as sucC and talB, in a host strain, wherein the highest yield increase rate can reach 60% or more. Currently, erythromycin is the most clear model compound in the study on the biosynthesis of polyketids. The production strain of the present invention enables massive accumulation of 6-deoxyerythronolide (6-dEB), an erythromycin precursor, in the fermentation process, laying the foundation for the industrial production of the heterologous synthesis of erythromycin by


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