The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 16, 2021

Filed:

Jun. 15, 2018
Applicant:

Ajinomoto Co., Inc., Tokyo, JP;

Inventors:

Kunihiro Hirai, Kawasaki, JP;

Satoshi Katayama, Kawasaki, JP;

Naoko Hirose, Kawasaki, JP;

Taisuke Ichimaru, Kawasaki, JP;

Ken Yamashita, Kawasaki, JP;

Daisuke Takahashi, Kawasaki, JP;

Assignee:

AJINOMOTO CO., INC., Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C07H 19/167 (2006.01); C07H 19/20 (2006.01); C07H 1/00 (2006.01); C07H 19/10 (2006.01); C07H 21/00 (2006.01); C07H 19/073 (2006.01); C07H 19/173 (2006.01); C07H 19/067 (2006.01);
U.S. Cl.
CPC ...
C07H 21/02 (2013.01); C07H 1/00 (2013.01); C07H 19/067 (2013.01); C07H 19/073 (2013.01); C07H 19/10 (2013.01); C07H 19/167 (2013.01); C07H 19/173 (2013.01); C07H 19/20 (2013.01); C07H 21/00 (2013.01); C07H 21/04 (2013.01); C12N 15/09 (2013.01);
Abstract

Oligonucleotides may be produced by a process, including (1) condensing a nucleoside, nucleotide or oligonucleotide (b), and a nucleoside, nucleotide or oligonucleotide (a), or a substituted nucleotide or oligonucleotide (α) in a non-polar solvent to give a reaction solution containing a phosphite triester product (c); (3) oxidizing or sulfurizing the phosphite triester product (c) to give a reaction solution containing an oligonucleotide (d) wherein the 5'-hydroxy group is protected; (4) deprotecting the oligonucleotide (d) to give a reaction solution containing an oligonucleotide (e) wherein the 5′-hydroxy group is not protected; and (6) adding a polar solvent to the reaction solution containing the oligonucleotide (e) and purifying the oligonucleotide (e) by solid-liquid separation, wherein said nucleoside, nucleotide or oligonucleotide (a) or said substituted nucleotide or oligonucleotide (α) is a compound represented by formula (a-i): wherein Base, R, R, m, L, Y, and Z are defined herein.


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