The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 09, 2021

Filed:

Mar. 05, 2019
Applicant:

Qingdao University, Qingdao, CN;

Inventors:

Rijun Gui, Qingdao, CN;

Yongxin Fu, Qingdao, CN;

Hui Jin, Qingdao, CN;

Xiangning Bu, Qingdao, CN;

Assignee:

QINGDAO UNIVERSITY, Qingdao, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C09K 11/06 (2006.01); C07D 311/78 (2006.01); C09K 11/58 (2006.01); G01N 21/64 (2006.01);
U.S. Cl.
CPC ...
C09K 11/06 (2013.01); C07D 311/78 (2013.01); C09K 11/58 (2013.01); G01N 21/643 (2013.01); C09K 2211/1007 (2013.01); C09K 2211/1029 (2013.01); G01N 2021/6432 (2013.01);
Abstract

A method for preparing a ratiometric fluorescent probe for melamine based on a DNA-stable silver nanocluster-rhodamine 6G complex, wherein the electrostatic self-assembly technology is adopted to construct a silver nanocluster-rhodamine 6G complex. The melamine forms a strong hydrogen bond with thymine in the DNA of the surface of the silver nanocluster, causing rhodamine 6G to dissociate from the surface of the silver nanocluster, destroying the fluorescence resonance energy transfer, so as to restore the fluorescence of the silver nanocluster. This process has little effect on Rhodamine 6G fluorescence which can be used as a reference signal, while silver nanocluster fluorescence can be used as a response signal. By fitting the linear relationship between the ratio of fluorescence emission peak intensities of the silver nanocluster to rhodamine 6G and the molar concentration of the melamine, the ratiometric fluorescent probe for melamine can be constructed.


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