The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 09, 2021

Filed:

May. 21, 2018
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Andrew Ronald Hagen, Oakland, CA (US);

Cheryl A. Kerfeld, Walnut Creek, CA (US);

Markus Sutter, Berkeley, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 14/195 (2006.01); C07K 14/245 (2006.01); C07K 14/47 (2006.01); C12P 21/06 (2006.01); C12P 21/02 (2006.01); C12N 15/62 (2006.01); C12N 11/04 (2006.01);
U.S. Cl.
CPC ...
C07K 14/195 (2013.01); C07K 14/245 (2013.01); C07K 14/47 (2013.01); C12N 11/04 (2013.01); C12N 15/62 (2013.01); C12P 21/02 (2013.01); C12P 21/06 (2013.01); C07K 2319/00 (2013.01); C07K 2319/21 (2013.01); C07K 2319/24 (2013.01); C07K 2319/50 (2013.01);
Abstract

The present disclosure is related to a BMC fusion protein that is capable of in vitro assembly, comprising a constituent BMC shell protein subunit and a sterically hindering protein domain that is cleavable. The BMC fusion protein is capable of in vitro assembly triggered by removal of the fused sterically hindering domain. The present disclosure is also related to a means to produce BMC shells in vitro, triggered by removal of a fused sterically hindering domain from one or more constituent BMC shell protein subunits. The BMC fusion protein enables encapsulation of broad classes of materials and biophysical studies of shell assembly, encapsulation, and permeability that would otherwise be unavailable from BMCs assembled in vivo.


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