The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 09, 2021

Filed:

Jun. 27, 2017
Applicant:

Biocytogen Pharmaceuticals (Beijing) Co., Ltd, Beijing, CN;

Inventors:

Yuelei Shen, Beijing, CN;

Yang Bai, Beijing, CN;

Rui Huang, Beijing, CN;

Xiaofei Zhou, Beijing, CN;

Yuting Hu, Beijing, CN;

Yanan Guo, Beijing, CN;

Jichao Du, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/027 (2006.01); G01N 33/00 (2006.01); C12N 15/00 (2006.01); A61D 19/04 (2006.01); A61K 49/00 (2006.01); C07K 14/705 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/85 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); A61D 19/04 (2013.01); A61K 49/0008 (2013.01); C07K 14/70521 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/8509 (2013.01); A01K 2217/072 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0331 (2013.01); C12N 2015/8572 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

Provided is a method for preparing a PD-1 gene-modified humanized animal model. The method utilizes the CRIPSR/Cas9 technique to replace partial fragments of a mouse PD-1 gene with fragments of a human PD-1 gene using homologous recombination by constructing a targeting vector, thereby preparing a gene-modified humanized mouse. This mouse can normally express a PD-1 protein containing the functional domain of the human PD-1 protein, and can be used as an animal model for mechanism research regarding PD-1, PD-L1 and other signals, for screening regulators, and for toxicological research. The method has an important and high application value in studies on functions of the PD-1 gene and in the development of new drugs.


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