The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 02, 2021

Filed:

Sep. 01, 2016
Applicant:

Creighton University, Omaha, NE (US);

Inventors:

Nancy D. Hanson, Omaha, NE (US);

Lucas Harrison, Omaha, NE (US);

Assignee:

Creighton University, Omaha, NE (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/689 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/689 (2013.01);
Abstract

Methods for differentiatingsequence type 131 are described. In one implementation, a method that employs example techniques in accordance with the present disclosure for determiningsequence type 131 includes amplifying a gene target in the presence of a fluorescent reporter dye; exposing the gene target and the fluorescent reporter dye to an increasing temperature gradient to denature and reduce the helicity of a double-stranded oligo of the gene target; recording fluctuations in fluorescence using a High Resolution Melting analysis (HRM)-capable thermocycler; and producing a melt curve unique to the gene target.


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