The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 26, 2021

Filed:

Mar. 17, 2017
Applicant:

University of Bath, Bath, GB;

Inventor:
Assignee:

UNIVERSITY OF BATH, Bath, GB;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/00 (2006.01); G01N 21/64 (2006.01); G16C 20/30 (2019.01); G16C 20/40 (2019.01);
U.S. Cl.
CPC ...
G01N 21/6486 (2013.01); G01N 21/64 (2013.01); G16C 20/30 (2019.02); G16C 20/40 (2019.02); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G01N 2021/6439 (2013.01);
Abstract

A protein structure analysis method includes receiving a fluorescence emission spectrum generated by a protein sample at a first excitation wavelength, evaluating, from the fluorescence emission spectrum, an indication characteristic of a fluorescence response of the protein sample at the first excitation wavelength, and repeating the receiving and evaluating steps in relation to a plurality of fluorescence emission spectra generated by the protein sample at a different excitation wavelength to the first excitation wavelength. In addition, the method includes generating a non-linear relationship between excitation wavelength and indication characteristic of fluorescence response of the protein sample that includes an indication of at least one characteristic of the conformational state of the protein sample. The method can be used with any protein which includes one or more naturally occurring fluorescent amino acids (intrinsic fluorophores), and particularly those with Trp residues (most), or proteins having appropriately selected extrinsic fluorophores.


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