The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 26, 2021

Filed:

Apr. 21, 2016
Applicant:

General Automation Lab Technologies, Inc., San Francisco, CA (US);

Inventors:

Paul C. Blainey, Cambridge, MA (US);

Michael W. Seely, San Diego, CA (US);

Roman Stocker, Zurich, CH;

Karsten Zengler, Cardiff by the Sea, CA (US);

Scott Conradson, Los Altos Hills, CA (US);

Peter Christey, San Francisco, CA (US);

Alexander Hallock, Redwood City, CA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07H 1/00 (2006.01); C12Q 1/6888 (2018.01); C12Q 1/6869 (2018.01); B01L 3/00 (2006.01); C12Q 1/6853 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/689 (2018.01); C12M 1/32 (2006.01); C12M 1/04 (2006.01); B01L 3/02 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); B01L 3/5085 (2013.01); C12M 23/12 (2013.01); C12M 23/24 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6816 (2013.01); B01L 3/0244 (2013.01); B01L 2200/025 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/12 (2013.01); B01L 2200/141 (2013.01); B01L 2300/021 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/165 (2013.01);
Abstract

A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.


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