The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 26, 2021
Filed:
Oct. 14, 2015
Applicant:
Research Development Foundation, Carson City, NV (US);
Inventors:
Assignee:
Research Development Foundation, Carson City, NV (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 21/00 (2006.01); G16B 30/00 (2019.01); C12Q 1/37 (2006.01); C12Q 1/48 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6869 (2018.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12P 21/005 (2013.01); C12Q 1/37 (2013.01); C12Q 1/485 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6869 (2013.01); C12Y 207/10001 (2013.01); C12Y 304/2207 (2013.01); G01N 33/5008 (2013.01); G16B 30/00 (2019.02); G01N 2333/91205 (2013.01); G01N 2333/96466 (2013.01);
Abstract
Provided are improved methods for identifying the substrate recognition specificity or activity of a protease, convertase (sortase), or kinase. In some embodiments, methods are provided for identifying the endogenous protease or convertase cleaving patterns (e.g., 'cleaveOme') inside the secretory pathway of a living cell. Select embodiments involve aspects of yeast endoplasmic reticulum sequestration screening and next generation sequencing. Methods of producing polypeptides in Kex2 knockout yeast are also provided.