The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 26, 2021
Filed:
Oct. 19, 2015
Nanjing Annoroad Gene Technology Co., Ltd, Nanjing, CN;
Annoroad Gene Technology (Beijing) Co., Ltd, Beijing, CN;
Xiaolin Li, Beijing, CN;
Jiezhong Zhang, Beijing, CN;
Hongmei Zhao, Beijing, CN;
Zhihua Pei, Beijing, CN;
Zhaoling Xuan, Beijing, CN;
Dawei Li, Beijing, CN;
Junbin Liang, Beijing, CN;
Chongjian Chen, Beijing, CN;
ZHEJIANG ANNOROAD BIO-TECHNOLOGY CO. LTD., Yiwu, CN;
ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD., Beijing, CN;
Abstract
Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.