The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 19, 2021
Filed:
Dec. 20, 2013
Hs Diagnomics Gmbh, Berlin, DE;
Julia-Marie Ritter, Berlin, DE;
Volkhard Seitz, Berlin, DE;
Steffen Hennig, Berlin, DE;
Michael Hummel, Berlin, DE;
HS DIAGNOMICS GMBH, Berlin, DE;
Abstract
Described herein is a method for amplifying a target nucleic acid sequence t-t-t-t-t' comprising a first amplification using a first primer pair with sequence m-K-pand m-K′-p′, and a subsequent second amplification using a second primer pair with sequence a-a-aand a′-a′-a′, wherein pis the same sequence as sequence element t. pand p′ are 8 to 40 nucleotides in length, K comprises a 3′-terminal sequence k1-k2-s, s is a mismatch sequences preventing PCR bias, ais the same sequence as sequence element k, a-ahybridize to a contiguous sequence on sequence element m-K, kis 2 to 9 nucleotides in length, aand a′ can be any sequence, and tis a variable region within the target nucleic acid sequence. Also described are collections of primer sets for use in the method of the invention.