The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 19, 2021

Filed:

Oct. 13, 2016
Applicant:

Japan Agency for Marine-earth Science and Technology, Yokosuka, JP;

Inventors:

Shunichi Urayama, Yokosuka, JP;

Takuro Nunoura, Yokosuka, JP;

Shigeru Deguchi, Yokosuka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6869 (2018.01); G16B 30/00 (2019.01); C12N 15/09 (2006.01); G01N 33/53 (2006.01); C12Q 1/68 (2018.01); G01N 33/569 (2006.01); C12N 7/02 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12N 7/00 (2006.01); C40B 50/06 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6869 (2013.01); C12N 7/02 (2013.01); C12N 15/09 (2013.01); C12N 15/1096 (2013.01); C12P 19/34 (2013.01); C12Q 1/68 (2013.01); G01N 33/53 (2013.01); G01N 33/569 (2013.01); G16B 30/00 (2019.02); C12N 7/00 (2013.01); C40B 50/06 (2013.01);
Abstract

The object is to provide a method that enables detection of unknown virus sequences and efficient detection and search of viruses. The method comprises the step of randomly fragmenting an objective double-stranded (ds) RNA to obtain dsRNA fragments; the step of subjecting the obtained dsRNA fragments to a reverse transcription reaction and then performing polymerase chain reaction (PCR) to obtain corresponding DNA fragments; and the step of subjecting the obtained DNA fragments to a sequence analysis operation to determine the sequences. The reverse transcription reaction is preferably started from the 3' ends of the dsRNA fragments.


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