The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 19, 2021
Filed:
Aug. 21, 2015
Applicant:
Cambridge Enterprise Limited, Cambridge, Cambridgeshire, GB;
Inventors:
Assignee:
CAMBRIDGE ENTERPRISE LIMITED, Cambridge, GB;
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/074 (2010.01); C12N 5/0735 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); C12N 5/0606 (2013.01); C12N 2500/38 (2013.01); C12N 2500/99 (2013.01); C12N 2501/065 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/15 (2013.01); C12N 2501/16 (2013.01); C12N 2501/235 (2013.01); C12N 2501/415 (2013.01); C12N 2501/60 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/724 (2013.01); C12N 2501/727 (2013.01); C12N 2501/73 (2013.01); C12N 2501/90 (2013.01); C12N 2501/999 (2013.01); C12N 2510/00 (2013.01); C12N 2533/52 (2013.01);
Abstract
The invention provides methods and materials for resetting or sustaining a human stem cell in a 'naïve' or 'ground' state, based on the use of media including combinations of inhibitors. An example naïve culture medium comprises a PKC inhibitor, a MEK inhibitor. Also provided are methods of obtaining or propagating such cells, cells obtained using these methods, and novel culture media, which can be used in these methods.