The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 05, 2021

Filed:

May. 30, 2016
Applicants:

Max-planck-gesellschaft Zur Förderung Der Wissenschaften E.v., Munich, DE;

Lead Discovery Center Gmbh, Dortmund, DE;

Inventors:

Tim Bergbrede, Dortmund, DE;

Nils-Göran Larsson, Cologne, DE;

Claes Gustafsson, Mölndal, SE;

Maria Falkenberg-Gustafsson, Mölndal, SE;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6818 (2018.01); C12Q 1/68 (2018.01); C12Q 1/6825 (2018.01); G01N 33/542 (2006.01); G01N 33/58 (2006.01); C12Q 1/48 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6818 (2013.01); C12Q 1/48 (2013.01); C12Q 1/68 (2013.01); C12Q 1/6825 (2013.01); G01N 33/542 (2013.01); G01N 33/582 (2013.01); G01N 2333/91245 (2013.01);
Abstract

The present invention relates to methods for detection of nucleotide polymerase activity and methods of detecting compounds that modulate nucleotide polymerase activity, by detecting product formation of the nucleotide polymerase to be tested based on determination of close proximity of two labeled nucleotide probes able to bind the product of the nucleotide polymerase. It is preferred that proximity dependent energy transfer, such as forster resonance energy transfer, between said labeled nucleotide probes is determined. The invention further provides kits comprising components for carrying out the inventive methods for detection of nucleotide polymerase activity.


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