The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 22, 2020

Filed:

Jun. 12, 2015
Applicant:

Momentum Bioscience, Ltd., Cardiff, GB;

Inventors:

Shawn Mark O'Hara, Richboro, PA (US);

Daniel Zweitzig, Feasterville, PA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6888 (2018.01); C12Q 1/04 (2006.01); C12Q 1/25 (2006.01); C12Q 1/42 (2006.01); C12Q 1/48 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6888 (2013.01); C12Q 1/04 (2013.01); C12Q 1/25 (2013.01); C12Q 1/42 (2013.01); C12Q 1/48 (2013.01);
Abstract

Methods for detecting microorganisms, in particular detection of bacteria and methods for measuring enzyme activity, such as Deoxyribonucleic acid (DNA) polymerase activity are disclosed. The aforesaid methods include, but are not limited to such methods performed on microbial crude lysates, useful for determining microbial enzyme activities, which can be linked to amplification signal generators such as real-time Polymerase Chain Reaction (PCR) techniques, thereby enabling determination of microbial pathogens in samples such as unpurified blood and other body fluids. Moreover, the disclosed embodiments also relate to reagents for use in such methods, and to test kits comprising such reagents for carrying out the methods.


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